Superhuman Protocols & Guide
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认知

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原作者：Dan Koe

For those on the cutting edge:

This document contains affiliate links to trusted sources for injectable, oral, or topical blends. I’ve personally spoke with the owners and verified the quality. They created the discount code “KOE” if you want 10% off.

If you have never used peptides before, read the “How To Reconstitute & Administer” section at the bottom of this cheatsheet.

You can search this document for tags and categories such as:

  • Fat loss
  • Healing
  • Longevity
  • Anti-aging
  • Immunity
  • Mitochondrial health
  • Muscle building
  • Cognition and focus
  • Appearance
  • Sexual wellness

I will add to this document as new formulations release, save it somewhere safe.

I am assuming that anyone looking at this document has a foundation of training and nutrition. These are not bandaids to root causes. They are enhancements to an already optimized routine. If you need a training program and nutrition education to start, consider the Superhuman Program.

Peptide & Supplement Research Cheat Sheet

DISCLAIMER

FOR RESEARCH AND EDUCATIONAL PURPOSES ONLY

The compounds listed in this document are primarily research chemicals and peptides that are NOT approved by the FDA for human consumption except where specifically noted. This information is provided solely for educational and research purposes. Many of these substances are sold with the explicit label “Not for Human Consumption” and are intended for laboratory research only.

This document does NOT constitute medical advice. Do not use any of these compounds without:

  • Consultation with a qualified healthcare provider
  • Proper medical supervision and monitoring
  • Understanding of the legal status in your jurisdiction
  • Laboratory testing and quality verification

The dosing protocols listed are derived from scientific literature, animal studies, and research trials – NOT medical prescriptions. Individual responses vary significantly. Risks may be understated or unknown due to limited human trials.

USE AT YOUR OWN RISK. THE AUTHOR ASSUMES NO LIABILITY.

My Favorite Peptides & Stacks

RETATRUTIDE

Categories: Fat Loss, Longevity, Mitochondrial Health

Description & Mechanism: Retatrutide is a triple agonist peptide targeting GIP (glucose-dependent insulinotropic polypeptide), GLP-1 (glucagon-like peptide-1), and glucagon receptors. It represents a next-generation approach beyond dual agonists like tirzepatide. By activating all three pathways, it influences glucose metabolism, insulin secretion, energy expenditure, and appetite regulation.

Science & Data: Phase 2 trials (NEJM, 2023) showed average weight loss of 24% at 48 weeks with the 12mg dose. The triple agonist approach appears more effective than GLP-1 alone or GIP/GLP-1 combinations. Glucagon receptor activation increases energy expenditure and promotes fat oxidation.

Main Risks:

  • Gastrointestinal distress (nausea, vomiting, diarrhea)
  • Potential thyroid C-cell tumors (black box warning class effect)
  • Gallbladder issues
  • Severe gastroparesis in rare cases
  • Rapid weight loss complications
  • Unknown long-term effects (still in trials)

Benefits:

  • Significant weight loss (superior to semaglutide)
  • Improved glycemic control
  • Reduced cardiovascular risk markers
  • Decreased liver fat
  • Improved lipid profiles
  • Potential metabolic health improvements
  • May preserve lean muscle better than GLP-1 alone

Dosing Protocols from Research:

Standard Protocol:

  • Start: 2mg subcutaneous split into 3 doses MWF
  • Titration: Increase by 1 mg every week
  • Sweet spot: 3-8mg weekly
  • Duration: Studies examined 48+ weeks

Microdosing Strategy (Experimental):

  • Start: 0.5-1mg subcutaneous 2-3x weekly
  • Titration: Increase by 0.25-0.5mg per injection every 2 weeks
  • Target: 2-4mg total per week split into multiple doses
  • Rationale: May reduce GI side effects while maintaining efficacy, though no clinical trials validate this approach

Links:

SLU-PP-332

Categories: Mitochondrial Health, Muscle Building, Longevity, Fat Loss

Description & Mechanism: A novel compound that acts as a Rev-Erbα/β agonist. It modulates circadian rhythm proteins and has shown remarkable effects on exercise capacity in animal models. It influences mitochondrial biogenesis, oxidative metabolism, and appears to mimic some benefits of exercise at the molecular level.

Science & Data: Mouse studies (Nature 2023) showed 70% increase in running endurance without exercise training, increased muscle fiber oxidative capacity, and improved metabolic markers. No human trials exist. Mechanisms include activation of mitochondrial biogenesis pathways and optimization of circadian metabolic rhythms.

Main Risks:

  • Only anecdotal human safety data exists
  • Potential circadian rhythm disruption
  • Unknown metabolic effects in humans
  • Possible sleep disturbances
  • Long-term safety unknown
  • Liver toxicity potential unknown

Benefits (Preclinical Only):

  • Dramatic increase in endurance capacity
  • Enhanced fat oxidation
  • Increased mitochondrial density
  • Improved oxidative metabolism
  • Potential metabolic health improvements
  • May enhance training adaptations

Dosing Protocols:

  • 250mcg orally 2-3 times per day
  • Increase dose to 500mg at your own risk

Links:

MOTS-C

Categories: Longevity, Mitochondrial Health, Fat Loss, Muscle Building, Anti-Aging

Description & Mechanism: A mitochondrial-derived peptide (MDP) encoded within mitochondrial DNA. It regulates metabolic homeostasis and cellular stress responses. MOTS-c improves insulin sensitivity, regulates mitochondrial function, and activates AMPK pathways. It represents a novel class of signaling molecules from mitochondria to nucleus.

Science & Data: Animal studies show improved glucose regulation, increased lifespan, enhanced exercise capacity, and protection against metabolic decline. Limited human studies show improved insulin sensitivity. Naturally declines with age. Activates skeletal muscle AMPK and targets the folate-methionine cycle.

Main Risks:

  • Limited long-term human safety data
  • Injection site reactions
  • Potential immune responses (peptide-based)
  • Flushing sensation reported
  • Unknown long-term effects
  • May affect folate metabolism

Benefits:

  • Improved insulin sensitivity
  • Enhanced metabolic flexibility
  • Increased exercise capacity
  • Potential lifespan extension
  • Protection against age-related metabolic decline
  • Improved mitochondrial function
  • May combat obesity and diabetes
  • Skeletal muscle benefits

Dosing Protocols:

  • 0.5-1.5mg subcutaneous injection
  • Frequency: 1x daily or on training days
  • Cycles: 8 weeks or longer

Links:

TADALAFIL

Categories: Sexual Wellness, Appearance (pump), Mitochondrial Health, Longevity

Description & Mechanism: A phosphodiesterase-5 (PDE5) inhibitor that increases cyclic GMP, causing smooth muscle relaxation and vasodilation. FDA-approved for erectile dysfunction, benign prostatic hyperplasia, and pulmonary arterial hypertension. Long half-life (17.5 hours) allows daily low-dose use.

Science & Data: Extensively studied with robust clinical data. Improves erectile function in 80%+ of men. Also shows cardiovascular benefits, potential neuroprotection, and exercise performance improvements. Daily 5mg showed cardiovascular benefits independent of ED treatment.

Main Risks:

  • Headache (most common)
  • Flushing
  • Dyspepsia
  • Back pain/muscle aches
  • Contraindicated with nitrates (dangerous interaction)
  • Vision changes (rare)
  • Priapism (very rare but serious)
  • Drop in blood pressure

Benefits:

  • Improved erectile function
  • Enhanced exercise capacity
  • Potential cardiovascular protection
  • Improved endothelial function
  • Reduced pulmonary hypertension
  • Potential neuroprotective effects
  • May improve muscle pump/blood flow
  • Daily use: spontaneity benefit
  • Possible longevity benefits via NO pathway

Dosing Protocols:

  • ED as-needed: 10-20mg taken 30-60 min before activity
  • ED daily: 2.5-5mg
  • Blood pressure: 5mg daily
  • Athletic/cardiovascular: 5-10mg daily
  • Studies support 2.5-20mg range depending on indication
  • For general benefits, stick to 5mg daily

Links:

METHYLENE BLUE

Categories: Cognition and Focus, Mitochondrial Health, Longevity, Anti-Aging

Description & Mechanism: A synthetic compound that acts as an alternative electron carrier in the mitochondrial electron transport chain. At low doses, it enhances mitochondrial respiration and acts as an antioxidant. It’s one of the oldest synthetic drugs and is FDA-approved for methemoglobinemia. Shows neuroprotective and cognitive-enhancing properties.

Science & Data: Low-dose studies (0.5-4mg/kg) show improved memory consolidation, enhanced mitochondrial function, and neuroprotection. Used clinically for over a century. Improves oxygen delivery to tissues and may slow cellular aging. Small human trials show cognitive benefits in healthy adults and potential for neurodegenerative diseases.

Main Risks:

  • Blue/green urine (expected, harmless)
  • SERIOUS: Serotonin syndrome risk with SSRIs, MAOIs
  • Contraindicated with G6PD deficiency
  • Nausea at higher doses
  • Dizziness
  • Potential hemolysis
  • Bladder irritation
  • Stains everything blue

Benefits:

  • Enhanced cognitive function and memory
  • Improved mitochondrial efficiency
  • Neuroprotection
  • Antimicrobial properties
  • Potential mood enhancement
  • May slow cellular aging
  • Improved oxygen utilization
  • Antioxidant at low doses
  • Potential Alzheimer’s/Parkinson’s benefit

Dosing Protocols:

  • Cognitive enhancement: 0.5-4mg/kg (35-280mg for 70kg person)
  • Common protocol: 10-20mg taken 1-2x daily
  • Pharmaceutical grade USP required
  • Start low (10mg) and assess tolerance
  • Studies used 15mg 3x daily or 280mg single dose
  • Cycling recommended (5 days on, 2 days off)

Links:

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DIHEXA

Categories: Cognition and Focus, Anti-Aging, Longevity

Description & Mechanism: A small molecule derived from angiotensin IV that potently enhances hepatocyte growth factor (HGF) binding to its receptor c-Met. Acts as a neurogenic and synaptogenic compound, promoting formation of new synapses. Shows remarkable cognitive enhancement in animal models. One of the most powerful nootropics in research.

Science & Data: Animal studies show dramatic improvements in learning and memory (7-fold better than reference compounds). Promotes synapse formation and neuronal connectivity. Extremely potent (milligram doses active). May reverse cognitive decline. Very limited human data but profound animal model results.

Main Risks:

  • MINIMAL human safety data exists
  • Potential for uncontrolled synapse formation
  • Unknown effects on brain development
  • Could theoretically affect cancer via HGF pathway
  • May cause overstimulation
  • Long-term consequences completely unknown
  • Possible headaches
  • No established safety profile

Benefits (Primarily Preclinical):

  • Profound cognitive enhancement
  • Improved learning and memory
  • Synapse formation and neuroplasticity
  • May reverse cognitive decline
  • Potential Alzheimer’s/dementia benefit
  • Enhanced neuronal connectivity
  • Possible stroke recovery aid
  • Long-lasting effects (persists after discontinuation)

Dosing Protocols: No established human protocols. Theoretical/reported usage:

  • Oral: 1-5mg daily
  • Animal studies suggest extremely low doses needed
  • Cycle: 1-2 weeks on, several weeks off
  • Some reports suggest 2-3mg daily
  • Effects may be long-lasting, requiring infrequent dosing
  • CAUTION: Very limited human experience

RADI-8 CREAM

Categories: Appearance, Anti-Aging

Description & Mechanism: A topical cosmetic blend marketed as a non-invasive alternative to botulinum toxin (botox) injections. The primary active ingredient is Snap-8 (Acetyl Octapeptide-3), a peptide that theoretically inhibits SNARE complex formation, reducing neurotransmitter release and muscle contraction depth. Combined with complementary skin-enhancing compounds: Vitamin C (collagen synthesis, antioxidant), Vitamin B3/Niacinamide (barrier function, anti-inflammatory), Vitamin E (antioxidant, membrane protection), Hyaluronic Acid (hydration, dermal plumping), and Kojic Acid (tyrosinase inhibitor for brightening).

Science & Data: Snap-8 studies show modest reduction in wrinkle depth (up to 30% in some trials) but effects are dramatically less than botulinum toxin. Works by topical penetration, not injection, limiting effectiveness. Individual components are well-studied: Niacinamide improves skin barrier (multiple RCTs), Vitamin C stimulates collagen (well-established), hyaluronic acid provides surface hydration (extensive cosmetic literature), and kojic acid inhibits melanin production (evidence-based for hyperpigmentation). The blend approach targets multiple aging mechanisms simultaneously.

Main Risks:

  • Generally very safe (topical application)
  • Potential skin irritation
  • Allergic reactions (especially to kojic acid)
  • Vitamin C can be unstable/irritating in some formulations
  • Kojic acid may cause contact dermatitis
  • Sun sensitivity (vitamin C and kojic acid)
  • Not suitable for all skin types
  • Eye irritation if applied too close

Benefits:

  • Reduction in fine lines and wrinkles (modest)
  • Non-invasive alternative to injectables
  • Improved skin texture and tone
  • Enhanced hydration
  • Brightening of hyperpigmentation
  • Antioxidant protection
  • Improved skin barrier function
  • Collagen synthesis support
  • No needles or downtime required
  • Cumulative benefits with consistent use
  • May reduce expression line depth

Dosing Protocols: Typical application guidelines:

  • Apply thin layer to clean, dry skin
  • Target areas: Forehead, around eyes, smile lines
  • Frequency: 1-2x daily (morning and/or evening)
  • Use consistently for 4-8 weeks to assess results
  • Apply before moisturizer, after cleansing
  • Use SPF during day (essential with vitamin C and kojic acid)
  • Avoid contact with eyes
  • Some protocols suggest twice-daily application for 60 days minimum
  • Effects are temporary and require ongoing use

Links:

GLOW BLEND

Categories: Appearance, Anti-Aging, Healing

Description & Mechanism: Not a standardized pharmaceutical compound – typically refers to custom peptide blends marketed for aesthetic benefits. Common formulations include combinations like GHK-Cu, BPC-157, and collagen peptides. Composition varies by provider. Aimed at skin, hair, and connective tissue.

Science & Data: No specific studies on “GLOW blend” as it’s not a defined compound. Efficacy depends entirely on components included. Individual peptides in such blends have varying levels of evidence. Marketing often outpaces science.

Main Risks:

  • Variable composition = unpredictable effects
  • Quality control issues
  • Risks of individual components apply
  • Potential interactions between peptides unknown
  • Allergic reactions
  • Injection site reactions

Benefits (Depend on Formulation):

  • May improve skin appearance
  • Potential collagen synthesis
  • Hair quality improvements
  • Nail strength
  • Wound healing support
  • Varies entirely based on ingredients

Dosing Protocols: Varies by manufacturer. Common protocols:

  • Subcutaneous injection daily
  • Typical blend may contain: GHK-Cu 2mg, BPC-157 250mcg per injection
  • Duration: 8-12 weeks
  • No standardized protocol exists

Links:

MELANOTAN 2 (MT2) {#melanotan-2-(mt2)}

Categories: Appearance, Sexual Wellness, Fat Loss

Description & Mechanism: A synthetic analog of alpha-melanocyte stimulating hormone (α-MSH). Binds to melanocortin receptors (MC1R and MC4R), inducing melanogenesis (tanning) and affecting sexual function, appetite, and inflammation. Originally developed for skin cancer prevention via tanning without UV exposure.

Science & Data: Induces tanning with minimal UV exposure in clinical trials. Also shows appetite suppression and enhanced erectile function/libido via MC4R activation. Limited formal human studies; development discontinued despite promising tanning effects. Commonly used off-label.

Main Risks:

  • Nausea (very common initially)
  • Flushing
  • Spontaneous erections (males)
  • Darkening of moles/freckles
  • New moles/freckles formation
  • Potential melanoma risk (theoretical but concerning)
  • Appetite suppression
  • Yawning/stretching syndrome
  • Unknown long-term safety

Benefits:

  • Skin tanning without UV exposure
  • Reduced sunburn risk
  • Enhanced libido and sexual function
  • Potential appetite suppression
  • Weight loss in some users
  • May reduce inflammation

Dosing Protocols:

  • Loading: 250-500mcg subcutaneous daily until desired tan achieved
  • Maintenance: 250-500mcg 1-2x weekly
  • Taken 30-60 min before sun/UV exposure for best results
  • Start low (100-250mcg) to assess tolerance
  • Typical loading: 7-14 days
  • Some protocols front-load 1mg first dose

Links:

BPC-157

Categories: Healing, Anti-Aging, Muscle Building, Appearance

Description & Mechanism: A synthetic peptide derived from body protection compound found in gastric juice. It promotes angiogenesis, modulates nitric oxide, interacts with the FAK-paxillin system, and shows broad tissue healing properties. Mechanism involves growth factor upregulation and anti-inflammatory pathways.

Science & Data: Extensive animal studies show accelerated healing of tendons, ligaments, muscles, and GI tract. Minimal human trials exist, but anecdotal reports are widespread. Studies show protection and healing of various tissues including gut, musculoskeletal, and neural. No FDA approval for any indication.

Main Risks:

  • NO FDA approval for human use
  • Limited human safety data
  • Potential angiogenesis concerns (theoretical cancer risk)
  • Injection site reactions
  • Unknown long-term effects
  • Possible immune system effects
  • Quality/purity varies widely between suppliers

Benefits (Mostly Preclinical):

  • Accelerated soft tissue healing
  • Tendon and ligament repair
  • Gut healing (leaky gut, ulcers)
  • Reduced inflammation
  • Neuroprotective properties
  • Improved joint health
  • Potential blood vessel formation
  • May protect against NSAID damage
  • Muscle healing acceleration

Dosing Protocols: Research and anecdotal protocols:

  • Subcutaneous: 250-500mcg twice daily
  • Oral: 500-1000mcg daily (unstable in stomach acid)
  • Typical duration: 4-8 weeks
  • Animal studies used 10mcg/kg equivalent doses

Links:

GHK-CU (COPPER PEPTIDE)

Categories: Healing, Anti-Aging, Appearance, Longevity

Description & Mechanism: A naturally occurring copper-binding peptide (Gly-His-Lys-Cu2+) that declines with age. It acts as a signaling molecule for tissue remodeling, anti-inflammatory responses, and antioxidant enzyme activation. Stimulates collagen/elastin production, modulates metalloproteinases, and influences gene expression related to healing.

Science & Data: Human skin studies show improved collagen synthesis, reduced fine lines, and improved wound healing. Decline from ~200ng/ml at age 20 to ~80ng/ml at age 60. Studies demonstrate increased collagen XVII, improved skin density, and anti-inflammatory effects. Used in cosmetic products extensively.

Main Risks:

  • Generally well-tolerated
  • Potential copper overload (theoretical with excessive use)
  • Injection site reactions
  • Possible allergic reactions
  • May affect wound remodeling if overdosed
  • Blue tint to skin at high concentrations

Benefits:

  • Improved skin appearance and elasticity
  • Enhanced collagen and elastin production
  • Wound healing acceleration
  • Reduced fine lines and wrinkles
  • Anti-inflammatory effects
  • Improved hair growth
  • Antioxidant properties
  • Tissue remodeling support
  • Potential anti-aging effects systemically

Dosing Protocols:

  • Topical: 1-3% concentration applied daily
  • Subcutaneous injection: 1-3mg 2-3x weekly
  • Oral: Poor bioavailability, not recommended
  • Typical duration: 8-12 weeks for cosmetic effects
  • Studies used 1-2mg/ml topical preparations
  • Injectable protocols: 2mg per injection, 3x weekly

Links:

TESAMORELIN

Categories: Fat Loss (visceral), Anti-Aging, Muscle Building, Longevity

Description & Mechanism: A synthetic growth hormone-releasing hormone (GHRH) analog that stimulates pituitary production of endogenous growth hormone. FDA-approved specifically for reducing excess abdominal fat in HIV patients with lipodystrophy. Maintains more physiological GH pulsatility compared to direct GH administration.

Science & Data: Clinical trials show 15-18% reduction in visceral adipose tissue (VAT) over 26 weeks. Improves IGF-1 levels, body composition, and may improve cognitive function. Unlike direct GH, lower risk of glucose dysregulation due to pulsatile release. Specific efficacy for dangerous visceral fat.

Main Risks:

  • Injection site reactions (common)
  • Joint pain
  • Potential glucose elevation
  • Increased risk of diabetes
  • Peripheral edema
  • May accelerate existing tumors (theoretical)
  • Contraindicated in active malignancy
  • Can cause carpal tunnel symptoms

Benefits:

  • Significant visceral fat reduction
  • Preserved lean muscle mass
  • Improved body composition
  • Potential cognitive benefits
  • Better lipid profiles
  • Increased IGF-1 levels
  • May improve skin quality
  • More physiological than direct GH

Dosing Protocols:

  • Standard: 2mg subcutaneous daily, before bed
  • Timing: Bedtime to align with natural GH pulse
  • Duration: Clinical trials were 26 weeks+
  • FDA-approved dose is 2mg daily
  • Some use 1mg daily to assess tolerance initially

Links:

5-AMINO-1MQ (Oral)

Categories: Fat Loss, Longevity, Mitochondrial Health

Description & Mechanism: A small molecule that inhibits nicotinamide N-methyltransferase (NNMT), an enzyme involved in NAD+ metabolism and energy regulation. By blocking NNMT, it theoretically increases NAD+ levels and activates SIRT1, influencing metabolic rate and fat storage.

Science & Data: Animal studies showed reduced fat accumulation and increased energy expenditure. Human data is extremely limited. Proposed mechanism suggests it activates similar pathways to caloric restriction by increasing NAD+ availability.

Main Risks:

  • Minimal human safety data
  • Potential hormonal effects unknown
  • Long-term metabolic consequences unclear
  • May affect methylation processes broadly
  • Injection site reactions (if injectable form)
  • Unknown drug interactions

Benefits (Mostly Theoretical):

  • Increased metabolic rate
  • Reduced fat storage
  • Potential NAD+ elevation
  • May support cellular energy metabolism
  • Possible anti-aging effects via SIRT1
  • Improved insulin sensitivity (in animals)

Dosing Protocols: Limited human data suggests:

  • Injectable: 50-100mg subcutaneous daily
  • Oral: 100-300mg daily
  • Typical duration: 30-60 days
  • Studies used 50mg daily in limited human trials

Links:

L-CARNITINE

Categories: Fat Loss, Mitochondrial Health, Muscle Building, Cognition and Focus

Description & Mechanism: An amino acid derivative essential for fatty acid transport into mitochondria for beta-oxidation. It shuttles long-chain fatty acids across the mitochondrial membrane where they can be burned for energy. Available as L-carnitine, acetyl-L-carnitine (crosses blood-brain barrier better), or L-carnitine L-tartrate.

Science & Data: Well-studied supplement with modest effects. Meta-analyses show 1-2 lb additional fat loss versus placebo over 12 weeks. More effective when combined with exercise. Acetyl-L-carnitine shows cognitive benefits in aging populations. Typical Western diet provides 60-180mg daily; vegetarians have lower levels.

Main Risks:

  • Generally very safe
  • Possible fishy body odor (TMAO production)
  • Mild GI upset at high doses
  • May interact with thyroid medications
  • Rare: nausea, vomiting at very high doses

Benefits:

  • Enhanced fat oxidation during exercise
  • Reduced muscle damage and soreness
  • Improved recovery
  • May improve insulin sensitivity
  • Brain health support (acetyl form)
  • Potential cardiovascular benefits
  • Enhanced sperm quality in men
  • Reduced fatigue

Dosing Protocols:

  • L-Carnitine: 2-3g daily, split into 2 doses with meals
  • Acetyl-L-Carnitine: 1-2g daily for cognitive benefits
  • Injectable L-Carnitine: 500-1000mg 2-3x weekly (higher bioavailability)
  • Take with carbohydrates for better uptake
  • Studies used 2-4g daily for 8-12 weeks

Links:

IPAMORELIN

Categories: Muscle Building, Anti-Aging, Fat Loss, Healing

Description & Mechanism: A selective growth hormone secretagogue (GHS) that binds to ghrelin receptors, stimulating GH release without significantly affecting cortisol or prolactin. More selective than older secretagogues like GHRP-6. Works synergistically with GHRH analogs by targeting different receptors.

Science & Data: Studies show increased GH and IGF-1 levels with minimal effect on other hormones. Safer profile than older secretagogues. Often combined with CJC-1295 (a GHRH analog) for synergistic effect. Limited long-term human studies but used extensively off-label.

Main Risks:

  • Injection site reactions
  • Water retention
  • Potential glucose dysregulation
  • Unknown long-term effects
  • May cause tiredness post-injection
  • Could exacerbate existing tumors
  • Hunger increase (mild)

Benefits:

  • Increased growth hormone production
  • Improved body composition
  • Enhanced recovery
  • Better sleep quality
  • Increased lean muscle
  • Reduced body fat
  • Improved skin quality
  • Accelerated healing
  • Minimal cortisol/prolactin effect

Dosing Protocols:

  • 200-300mcg subcutaneous injection
  • Frequency: 1-3x daily (before meals and/or bedtime)
  • Often dosed 2x daily: morning and pre-bed
  • Typical cycle: 12-16 weeks, then break
  • Maximum benefit when combined with GHRH analog
  • Studies used 200mcg 2-3x daily

Links:

GW501516 (CARDARINE)

Categories: Fat Loss, Mitochondrial Health, Muscle Building (endurance)

Description & Mechanism: A PPAR-δ (peroxisome proliferator-activated receptor delta) agonist that influences fat metabolism, energy expenditure, and endurance capacity. NOT actually a SARM despite marketing. Increases fatty acid oxidation, mitochondrial biogenesis, and exercise capacity. Development was discontinued due to cancer concerns in animal studies.

Science & Data: Studies showed improved endurance (up to 70% in mice), increased fat oxidation, improved lipid profiles. Human trials were terminated due to carcinogenicity in rodent studies at high doses over long periods. Some evidence for cardiovascular benefits at lower doses shorter term. No FDA approval.

Main Risks:

  • CANCER: Rapid tumor development in animal studies
  • Liver toxicity potential
  • NO long-term human safety data
  • Cardiovascular effects unclear in humans
  • May affect cell proliferation
  • Legal status: Banned by WADA for athletes
  • Quality/purity issues in black market sources

Benefits (Preclinical/Short-term Human):

  • Dramatic endurance increase
  • Enhanced fat oxidation
  • Improved lipid profiles
  • Increased mitochondrial density
  • May improve insulin sensitivity
  • Lean muscle preservation during cutting
  • Enhanced recovery

Dosing Protocols (From Limited Research):

  • 10-20mg oral daily
  • Studies used 2.5-10mg in humans (short duration)
  • Typical cycle: 8-12 weeks maximum (if used at all)
  • Half-life: 16-24 hours, once daily dosing

Links:

HCG (HUMAN CHORIONIC GONADOTROPIN)

Categories: Sexual Wellness, Muscle Building, Longevity

Description & Mechanism: A glycoprotein hormone that mimics luteinizing hormone (LH). In males, it stimulates testosterone production by Leydig cells. FDA-approved for hypogonadism and cryptorchidism. Used off-label during/after anabolic steroid cycles to maintain testicular function and fertility.

Science & Data: Well-established mechanism and efficacy. Maintains testicular size and function during testosterone suppression. Preserves fertility. Used medically for over 50 years. Efficacy well-documented in clinical trials for hypogonadism.

Main Risks:

  • Gynecomastia (converts to estrogen)
  • Water retention
  • Mood changes
  • Acne
  • Potential desensitization with overuse
  • May mask hypogonadism
  • Headaches
  • Injection site reactions

Benefits:

  • Maintains testicular size and function
  • Preserves fertility during TRT
  • Increases testosterone production
  • Prevents testicular atrophy
  • May improve mood/libido
  • Maintains intra-testicular testosterone
  • Supports LH receptor sensitivity

Dosing Protocols:

  • Fertility maintenance during TRT: 250-500 IU 2-3x weekly

Links:

NAD+ (NICOTINAMIDE ADENINE DINUCLEOTIDE)

Categories: Longevity, Anti-Aging, Mitochondrial Health, Cognition and Focus

Description & Mechanism: A critical coenzyme in every cell, involved in energy metabolism, DNA repair, and cellular signaling. Exists in oxidized (NAD+) and reduced (NADH) forms. Required for mitochondrial function and sirtuin activation. Levels decline with age. Administered IV or subcutaneously as it’s not orally bioavailable in full form.

Science & Data: Well-established critical role in metabolism. IV NAD+ shows acute benefits in energy and cognition. Decline with age is well-documented. Direct NAD+ administration bypasses the salvage pathway used by precursors. Small studies show benefits for addiction, fatigue, and cognitive function.

Main Risks:

  • IV administration: requires medical supervision
  • Nausea during infusion (common)
  • Flushing
  • Cramping
  • Chest tightness (if infused too rapidly)
  • Expensive
  • Requires slow infusion (2-4+ hours)

Benefits:

  • Immediate increase in NAD+ levels
  • Enhanced energy and mental clarity
  • May support DNA repair
  • Potential anti-aging effects
  • Improved mitochondrial function
  • Addiction recovery support (some evidence)
  • Enhanced cellular metabolism
  • Potential neuroprotection

Dosing Protocols:

  • Starting dose: 25-50mg subcutaneous daily
  • Standard dose: 50-100mg SubQ daily or every other day
  • Higher dose: 100-200mg IM 2-3x weekly
  • Split dosing option: 50mg twice daily for stable levels
  • Cycle strategy: Daily for 2-4 weeks, then 3x weekly maintenance

Links:

NMN (NICOTINAMIDE MONONUCLEOTIDE)

Categories: Longevity, Anti-Aging, Mitochondrial Health, Cognition and Focus

Description & Mechanism: A precursor to NAD+ that enters cells more efficiently than other precursors. Converted to NAD+ through the salvage pathway. Oral bioavailability appears better than NAD+ itself. May have direct signaling functions beyond NAD+ generation. Alternative to NR (nicotinamide riboside).

Science & Data: Animal studies show impressive anti-aging effects, improved metabolism, and enhanced NAD+ levels. Human trials show increased NAD+ levels, improved insulin sensitivity, and muscle function. More human data emerging. Appears safe and well-tolerated. May be superior to NR for some individuals.

Main Risks:

  • Very safe profile in studies to date
  • Mild GI upset possible
  • Flushing (rare, at high doses)
  • Long-term effects still being studied
  • Quality varies between suppliers
  • Potential methylation demands (theoretical)

Benefits:

  • Increased NAD+ levels
  • Improved mitochondrial function
  • Enhanced insulin sensitivity
  • Potential anti-aging effects
  • Improved muscle function
  • Better endurance
  • Cardiovascular benefits
  • May improve cognitive function
  • Supports DNA repair

Dosing Protocols:

  • Common: 250-1000mg oral daily
  • Studies used 250-500mg daily
  • Take in morning (may affect sleep if taken at night)
  • Sublingual absorption may be superior
  • Some protocols use 1000mg divided into 2 doses
  • Typical range: 500-1000mg daily for anti-aging

Links:

SELANK

Categories: Cognition and Focus, Immunity, Anti-Aging

Description & Mechanism: A synthetic heptapeptide derived from tuftsin (immune system peptide). Acts as an anxiolytic by modulating GABA and serotonin, while also affecting BDNF (brain-derived neurotrophic factor). Developed in Russia for anxiety and cognitive enhancement. Modulates enkephalins and affects monoamine metabolism.

Science & Data: Russian studies show anxiolytic effects without sedation, improved learning and memory, and immune modulation. Limited Western clinical trials. Appears to stabilize enkephalins, affecting anxiety and mood. Neuroprotective properties demonstrated in research. Used in Russia as approved medication.

Main Risks:

  • Limited safety data outside Russia
  • Potential immune system effects
  • Headaches
  • Nasal irritation (intranasal form)
  • Fatigue in some users
  • Unknown long-term effects
  • Injection site reactions

Benefits:

  • Reduced anxiety without sedation
  • Improved focus and concentration
  • Enhanced learning and memory
  • Immune system support
  • Neuroprotective effects
  • Improved mood
  • No addiction potential
  • May reduce depression symptoms

Dosing Protocols:

  • Intranasal: 100-300mcg 2-3x daily
  • Subcutaneous: 250-500mcg daily
  • Typical cycle: 2-4 weeks, then break
  • Studies used 100-300mcg 2-3x daily intranasal
  • Effects build over several days of use

Links:

SEMAX

Description & Mechanism: A synthetic heptapeptide analog of ACTH (adrenocorticotropic hormone) fragment 4-10. Developed in Russia for cognitive enhancement and neuroprotection. Increases BDNF, modulates neurotransmitters (dopamine, serotonin, acetylcholine), and protects against hypoxia and oxidative stress.

Categories: Cognition and Focus, Anti-Aging, Longevity

Science & Data: Russian studies show cognitive enhancement, neuroprotection, and improved recovery from stroke. Increases BDNF levels significantly. Improves attention, memory, and mental clarity. Used medically in Russia for stroke recovery and cognitive disorders. Limited Western trials.

Main Risks:

  • Limited safety data outside Russia
  • Potential headaches
  • Nasal irritation (intranasal form)
  • Overstimulation in some users
  • May affect sleep if taken late
  • Unknown long-term effects
  • Injection site reactions

Benefits:

  • Enhanced cognitive function
  • Improved focus and concentration
  • Increased mental energy
  • Neuroprotection
  • Enhanced learning and memory
  • Potential antidepressant effects
  • Increased BDNF levels
  • May accelerate stroke recovery
  • Mood improvement

Dosing Protocols:

  • Intranasal: 300-600mcg 2-3x daily
  • Subcutaneous: 250-500mcg daily
  • Common: 600mcg divided into 2 doses (intranasal)
  • Effects are relatively acute (within hours)
  • Cycle: 2-4 weeks use, 1-2 weeks off
  • Studies used 300-1200mcg daily intranasal

Links:

SR-9011

Categories: Mitochondrial Health, Fat Loss, Muscle Building, Longevity

Description & Mechanism: A Rev-Erbα/β agonist similar to SR-9009 but with improved bioavailability. Modulates circadian rhythm proteins, influences metabolic genes, and affects mitochondrial function and fat metabolism. Theoretical “exercise mimetic” that alters metabolism at the genetic level.

Science & Data: Primarily preclinical data. Shows increased metabolic rate, enhanced endurance, and fat loss in animal studies. Alters circadian metabolism genes. Better oral bioavailability than SR-9009. No published human trials. Modulates metabolic gene expression in skeletal muscle.

Main Risks:

  • NO human safety data
  • Potential circadian disruption
  • Unknown metabolic consequences
  • May affect sleep patterns
  • Long-term effects completely unknown
  • Quality/purity concerns in research market
  • Potential liver toxicity unknown

Benefits (Preclinical Only):

  • Increased metabolic rate
  • Enhanced endurance capacity
  • Improved fat oxidation
  • Better mitochondrial function
  • Potential muscle endurance improvement
  • May not suppress natural circadian rhythms (vs SR-9009)
  • Enhanced exercise capacity without exercise

Dosing Protocols: No established human protocols. Speculative based on animal studies:

  • Oral: 5-20mg daily
  • May be split into 2 doses
  • Animal studies suggest 20-100mg/kg would extrapolate to ~140-700mg for 70kg human (likely excessive)
  • Cycle: Theoretical 8-12 weeks maximum

Links:

SS-31 (ELAMIPRETIDE) {#ss-31-(elamipretide)}

Categories: Mitochondrial Health, Longevity, Anti-Aging, Healing

Description & Mechanism: A mitochondria-targeting peptide that concentrates in the inner mitochondrial membrane. Binds to cardiolipin, stabilizing cristae structure and optimizing electron transport chain function. Reduces reactive oxygen species (ROS) and improves mitochondrial energetics. Undergoing clinical trials for mitochondrial diseases and heart failure.

Science & Data: Phase 2/3 trials for various conditions (heart failure, mitochondrial myopathy). Shows improved mitochondrial function, reduced oxidative stress, and tissue protection. Human studies demonstrate improved exercise capacity and cardiac function. Represents novel approach to aging via mitochondrial optimization.

Main Risks:

  • Still in clinical development
  • Limited long-term human data
  • Injection site reactions
  • Potential immune responses
  • Unknown effects of chronic use
  • Expensive and limited availability
  • Diarrhea reported in trials

Benefits:

  • Improved mitochondrial efficiency
  • Enhanced cellular energy production
  • Reduced oxidative stress
  • Potential cardiac protection
  • Improved exercise capacity in mitochondrial disease
  • May slow cellular aging
  • Tissue protection during ischemia
  • Enhanced organ function

Dosing Protocols: Clinical trials used:

  • Subcutaneous: 40mg daily
  • IV infusion: 0.25mg/kg (17.5mg for 70kg)
  • Studies examined 28 days to 6 months
  • Some trials used 40mg subcutaneous once daily
  • Duration: Clinical trials 4-24 weeks

GLUTATHIONE (INTRAMUSCULAR)

Categories: Longevity, Anti-Aging, Immunity, Appearance, Mitochondrial Health

Description & Mechanism: A tripeptide (L-glutamine, L-cysteine, L-glycine) and the body’s master antioxidant. Synthesized intracellularly and crucial for neutralizing reactive oxygen species, detoxification (Phase II conjugation), immune function, and DNA synthesis/repair. IM administration provides slower, sustained absorption compared to IV, with potential for depot effect in muscle tissue. Glutathione acts intracellularly where IV bolus dosing may result in rapid metabolism before cellular uptake.

Science & Data: Well-established as critical antioxidant system. Levels decline with age, illness, and oxidative stress. IV glutathione shows rapid plasma elevation but quick clearance; IM may provide more sustained tissue availability. Studies on supplementation show improved oxidative stress markers, though efficacy debates exist regarding absorption. Skin lightening effects are documented but controversial. Parenteral administration bypasses GI degradation (oral bioavailability is poor). Some evidence for neuroprotection and liver support.

Main Risks:

  • Injection site reactions (pain, swelling, redness)
  • Potential allergic reactions (rare)
  • Possible zinc depletion with chronic high-dose use
  • May lower blood pressure in some individuals
  • Skin lightening (desired by some, unwanted by others)
  • Potential interaction with chemotherapy drugs
  • Abdominal cramping (rare)
  • Risk of infection with any IM injection
  • Uneven skin lightening possible

Benefits:

  • Enhanced antioxidant defense system
  • Improved detoxification capacity
  • Liver support and protection
  • Skin brightening and tone evening
  • Reduced hyperpigmentation
  • Anti-aging effects (cellular level)
  • Immune system support
  • May improve energy levels
  • Potential neuroprotection
  • Reduced oxidative stress markers
  • May improve insulin sensitivity
  • Support for heavy metal detoxification
  • Enhanced recovery
  • Possible reduction in fine lines

Dosing Protocols: IM administration guidelines:

  • Common dose: 600-1200mg per injection
  • Frequency: 1-3x weekly
  • Typical sites: Deltoid or gluteal muscle
  • Popular protocols:
    • Maintenance: 600mg 1-2x weekly
    • Intensive: 1200mg 2-3x weekly for 4-8 weeks
    • Some use 1200mg 3x weekly initially, then taper to weekly
  • Usually combined with Vitamin C (500-1000mg) for synergy
  • Cycle duration: 8-12 weeks intensive, then maintenance
  • Injection volume: Typically 2-4mL depending on concentration
  • Studies examining skin effects used 600mg 2x weekly for 12 weeks
  • Sustained release from IM depot may provide advantages over IV

Links:

RETATRUTIDE + CAGRILINTIDE COMBINATION

Categories: Fat Loss, Longevity

Description & Mechanism: Combines retatrutide (GIP/GLP-1/glucagon triple agonist) with cagrilintide (long-acting amylin analog). Amylin is co-secreted with insulin and slows gastric emptying, reduces glucagon, and promotes satiety through different mechanisms than GLP-1.

Science & Data: Theoretical synergy based on complementary mechanisms. Cagrilintide alone showed 10.8% weight loss at 2.4mg weekly (NEJM 2021). The combination has not been studied in formal trials but represents multi-pathway appetite and metabolic regulation.

Main Risks:

  • Additive GI side effects (severe nausea risk)
  • Hypoglycemia (especially with insulin)
  • Severe gastroparesis risk increased
  • All risks from retatrutide
  • Injection site reactions
  • Potential drug interactions unknown

Benefits:

  • Potentially greater weight loss than either alone
  • Multi-mechanism metabolic improvement
  • Complementary appetite suppression
  • May help weight loss plateaus
  • Different satiety signaling pathways

Dosing Protocols: No formal protocols exist. Experimental approaches used in clinical practice:

  • Cagrilintide: 0.6-2.4mg weekly subcutaneous
  • Retatrutide: 4-8mg weekly subcutaneous
  • Typically stagger dose escalation to assess tolerance
  • Consider alternating injection days
  • Start LOWER than you would on Retatrutide

Links:

  • Retatrutide + Cagrilinitide 20mg – contains 10mg of retatrutide and 10mg of cagrilinitide. Reconstitute with 2mg of bacteriostatic water so that 10 units (0.1mL) equals 1mg of both.

HOW TO RECONSTITUTE AND ADMINISTER PEPTIDES

SUPPLIES YOU’LL NEED

For Reconstitution:

  • Lyophilized peptide vial (powder form)
  • Bacteriostatic water (0.9% benzyl alcohol)
  • Alcohol swabs
  • Syringe (3mL or 5mL with needle for drawing)
  • Or insulin syringe (use it multiple times if you need more than 1mL of bacteriostatic water)
  • Storage: Refrigerator (2-8°C)

For Injection:

STEP 1: RECONSTITUTING PEPTIDES

Understanding Reconstitution: Your peptide arrives as a freeze-dried powder (lyophilized). You must add bacteriostatic water to dissolve it before use. The amount of water you add determines the concentration and how much you’ll inject each time.

Calculating How Much Water to Add:

Let’s use an example: You have 5mg of BPC-157 and want each injection to be 250mcg (0.25mg).

  1. Decide your dose per injection: 250mcg = 0.25mg
  2. Calculate total doses in vial: 5mg ÷ 0.25mg = 20 doses
  3. Choose convenient volume per dose: 0.25mL (25 units on insulin syringe) is easy to measure
  4. Calculate total water needed: 20 doses × 0.25mL = 5mL bacteriostatic water

Quick Reference Formula:

  • If you want each 0.1mL (10 units) to equal your dose: Add 1mL water per 1mg of peptide
    • Most peptides come in a 10mg vial. If you add 1mL of bacteriostatic water, then the 10 unit mark (0.1mL) will equal 1 milligram.
  • If you want each 0.2mL (20 units) to equal your dose: Add 2mL water per 1mg of peptide
  • If you want each 0.25mL (25 units) to equal your dose: Add 2.5mL water per 1mg of peptide

The Reconstitution Process:

  1. Prepare your workspace:
    • Wash hands thoroughly
    • Clean surface with alcohol wipe
    • Gather all supplies
  2. Wipe the tops:
    • Remove plastic caps from peptide vial and bacteriostatic water
    • Wipe both rubber stoppers with alcohol swabs
    • Let air dry 10-15 seconds
  3. Draw the bacteriostatic water:
    • Attach needle to 3mL syringe
    • Insert needle into bacteriostatic water vial
    • Pull back plunger to draw calculated amount (e.g., 2mL)
    • Remove any air bubbles by tapping syringe and pushing air out
  4. Add water to peptide (CRITICAL TECHNIQUE):
    • Insert needle into peptide vial at an angle
    • DO NOT spray directly onto powder
    • Aim the stream at the glass wall of the vial
    • Let the water slowly run down the side
    • Inject slowly and gently
    • Never shake the vial – this can damage peptides
  5. Dissolve the peptide:
    • Once all water is added, gently swirl (don’t shake)
    • Let sit in refrigerator for 5-10 minutes
    • Gently roll vial between hands if needed
    • Solution should be clear (some peptides may be slightly cloudy)
    • If powder remains, wait longer or gently roll more
  6. Label and store:
    • Write date of reconstitution on vial
    • Store in refrigerator (2-8°C)
    • Use within 30 days
    • Keep away from light (some peptides are light-sensitive)

STEP 2: DRAWING YOUR DOSE

  1. Prepare the insulin syringe:
    • Use a fresh insulin syringe (29-31 gauge, 0.3-1mL size)
    • Do not remove cap yet
  2. Wipe the vial:
    • Clean rubber stopper with alcohol swab
    • Let dry 10-15 seconds
  3. Draw air equal to your dose:
    • Remove syringe cap
    • Pull plunger back to draw air equal to your dose
    • Example: If dosing 0.25mL (25 units), draw 0.25mL of air
  4. Inject air into vial:
    • Insert needle into vial
    • Push air in (this prevents vacuum)
  5. Draw your dose:
    • Turn vial upside down with needle inserted
    • Pull plunger back to slightly past your dose
    • Tap syringe to move air bubbles to top
    • Push plunger to expel air and reach exact dose
    • Remove needle from vial
  6. Recap carefully:
    • Carefully recap needle (or use safety cap if provided)
    • Set aside on clean surface

STEP 3: CHOOSING INJECTION SITES

Best Subcutaneous Sites (From Easiest to Advanced):

  1. Lower Abdomen (RECOMMENDED FOR BEGINNERS):
    • Best area: 2 inches away from belly button in all directions
    • Avoid: Area directly around navel
    • Why it’s best: Easy to reach, lots of subcutaneous fat, less painful
    • Imagine a clock around your belly button – rotate between 2, 4, 8, and 10 o’clock positions
  2. Upper Thigh:
    • Outer or middle front of thigh
    • Top half of thigh (not near knee)
    • Good alternative if abdomen is overused
  3. Love Handles/Flanks:
    • Side of abdomen above hip bone
    • Pinch and inject into fat roll
  4. Upper Arm (Harder – May Need Help):
    • Back of upper arm
    • Difficult to reach and inject yourself

Rotation Strategy:

  • Never inject same spot twice in a row
  • Wait at least 1 week before using same exact spot
  • Keep a mental or written log of rotation
  • Example rotation: Monday = right lower abdomen, Wednesday = left lower abdomen, Friday = right thigh, etc.

STEP 4: THE INJECTION TECHNIQUE

Preparation:

  1. Clean injection site:
    • Wipe area with alcohol swab
    • Clean in circular motion outward
    • Let air dry completely (30 seconds) – wet alcohol stings
  2. Position yourself:
    • Sit or stand comfortably
    • Good lighting
    • Relax – tension makes it more painful

The “Pinch and Poke” Method:

  1. Pinch the skin:
    • Use thumb and forefinger of non-dominant hand
    • Grab a fold of skin and fat (about 1-2 inches of tissue)
    • Pinch firmly but not painfully
    • This lifts subcutaneous fat away from muscle
    • Why: Ensures you inject into fat, not muscle
  2. Hold the syringe:
    • Hold like a dart with dominant hand
    • Remove cap
  3. Insert the needle:
    • Angle: 45-90 degrees (90° for more fat, 45° for leaner areas)
    • Quick, smooth motion – don’t hesitate
    • Insert entire needle in one motion
    • You should feel slight pressure but minimal pain
  4. Inject the medication:
    • Keep pinching the skin
    • Slowly push plunger down (3-5 seconds)
    • Don’t rush – rapid injection can cause discomfort
  5. Remove and dispose:
    • Quickly pull needle straight out
    • Release skin pinch
    • Apply gentle pressure with cotton ball if needed (don’t rub)
    • Immediately place syringe in sharps container
    • Never recap a used needle

If You See Blood:

  • Small drop is normal – you hit a capillary
  • Apply pressure for 30 seconds
  • Not a problem, just cosmetic

If Injection Hurts:

  • Stop if severe pain (may have hit muscle)
  • Withdraw, choose new site
  • Ensure proper pinch technique

STEP 5: COMBINING MULTIPLE PEPTIDES (ADVANCED)

When You Can Combine:

  • Multiple peptides with same dosing schedule
  • Compatible pH and reconstitution
  • Example: BPC-157 + TB-500 in one injection

Method 1: Drawing Multiple Peptides into One Syringe (Frontloading)

  1. Calculate total volume:
    • Peptide A dose: 0.2mL
    • Peptide B dose: 0.25mL
    • Total: 0.45mL (make sure it fits in your syringe)
  2. Draw first peptide:
    • Clean vial A
    • Draw air, inject into vial
    • Draw Peptide A dose (0.2mL)
  3. Draw second peptide:
    • Clean vial B
    • Draw additional air needed
    • Inject into vial B
    • Draw Peptide B dose (0.25mL)
    • You now have 0.45mL total in syringe
  4. Inject as normal

Method 2: Backloading (For Insulin Syringes)

Insulin syringes have fixed needles, making Method 1 difficult. Backloading solves this:

  1. Draw peptides using separate syringe:
    • Use 3mL syringe with removable needle
    • Draw all peptides into this syringe (combined dose)
  2. Remove plunger from insulin syringe:
    • Pull plunger completely out from back of insulin syringe barrel
    • Set plunger aside on clean surface
  3. Transfer peptides:
    • Insert 3mL syringe tip into back of insulin syringe barrel
    • Push peptide solution into barrel
    • Remove 3mL syringe
  4. Replace plunger:
    • Carefully insert plunger back into barrel from back
    • Push in slowly until it contacts the liquid
    • Express any air bubbles
  5. Inject normally

Method 3: Pre-mixing in One Vial (Most Convenient)

For peptides you use together regularly:

  1. Reconstitute both peptides separately first
  2. Calculate combined dosing:
    • If mixing 5mg BPC-157 + 5mg TB-500
    • You’ll draw proportional amounts from each to get desired ratio
  3. Some advanced users draw from both vials into a sterile vial:
    • Requires sterile technique
    • Not recommended for beginners
    • Risk of contamination

Safety Notes for Combining:

  • Only combine peptides you use simultaneously
  • Never mix compounds with different storage requirements
  • Start with single peptides until comfortable
  • Some peptides should NOT be combined (check compatibility)
  • When in doubt, use separate injections

STORAGE AND HANDLING

Before Reconstitution:

  • Store in cool, dry place or refrigerator
  • Avoid extreme temperatures
  • Can last months to years if properly stored

After Reconstitution:

  • Always refrigerate (2-8°C / 36-46°F)
  • Store in original vial
  • Keep away from light (some peptides degrade)
  • Use within 30 days (some stable longer, but 30 days is safe guideline)
  • Do not freeze

Signs of Degradation:

  • Solution becomes cloudy (when should be clear)
  • Color change
  • Particulates or floating material
  • Loss of effectiveness
  • When in doubt, discard

SAFETY AND TROUBLESHOOTING

Preventing Infections:

  • Always use alcohol swabs
  • Never reuse needles or syringes
  • Don’t touch needle tip
  • If needle touches anything non-sterile, discard
  • Watch for signs of infection: redness, warmth, swelling, pus

Injection Site Reactions:

  • Mild redness/swelling: Normal, usually resolves in hours
  • Itching: Can apply ice, take antihistamine if severe
  • Lumps: Usually from injecting too shallow or too fast
  • Bruising: Hit a blood vessel – not dangerous, just unsightly

Reducing Pain:

  • Let alcohol dry completely
  • Inject slowly
  • Use smallest gauge needle (31G less painful than 27G)
  • Relax muscles
  • Ice area for 30 seconds before (numbing)
  • Room temperature peptides (not cold from fridge) hurt less

Lipohypertrophy Prevention:

  • Always rotate sites
  • Don’t inject same spot more than once/week
  • Lumps from repeated injections take months to resolve
  • Can prevent absorption

Disposal:

  • Use sharps container (buy at pharmacy)
  • When 3/4 full, seal and dispose per local regulations
  • Many pharmacies accept sharps containers
  • Never throw loose needles in trash

QUALITY AND LEGAL CONSIDERATIONS

Peptide Quality:

  • Third-party testing (HPLC, mass spectrometry) essential
  • Request Certificates of Analysis (COA)
  • Research chemical market has quality/purity issues
  • Pharmaceutical grade when available always preferred
  • Properly labeled vials with:
    • Peptide name
    • Amount (mg)
    • Lot number
    • Expiration date

Legal Status:

  • Many peptides exist in regulatory gray areas
  • Not FDA-approved for most uses listed here
  • Often sold as “research chemicals” = “not for human consumption”
  • Consult local regulations before purchasing
  • Some peptides are controlled substances in certain jurisdictions

Medical Supervision:

  • Ideally work with knowledgeable healthcare provider
  • Regular bloodwork to monitor effects
  • Document your protocol and response
  • Report adverse reactions

BEGINNER’S FIRST TIME CHECKLIST

Before Your First Injection:

  • Read all instructions completely
  • Wash hands thoroughly
  • Gather all supplies
  • Calculate reconstitution volume
  • Choose injection site (recommend lower abdomen)
  • Have sharps container ready
  • Set up clean workspace

During Reconstitution:

  • Clean both vial tops
  • Draw bacteriostatic water
  • Inject water slowly down side of vial (not on powder)
  • Let dissolve without shaking
  • Label vial with date
  • Store in refrigerator

For Injection:

  • Draw your dose carefully
  • Clean injection site and let dry
  • Pinch skin firmly
  • Insert needle quick and smooth (don’t hesitate)
  • Inject slowly over 3-5 seconds
  • Remove needle and dispose immediately
  • Document: date, time, dose, location

After Injection:

  • Return peptide to refrigerator
  • Note injection site for rotation
  • Monitor for any adverse reactions
  • Track results/effects in journal

Remember: Take your time, don’t rush, and it gets easier with practice. The first injection is the hardest mentally. By your 5th injection, it will feel routine.

Remember: This is for educational and research purposes only. Consult healthcare professionals before considering any of these compounds.

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